View predicted electrophoresis patterns of restriction digests including partial digests.Cut and paste DNA segments from one construct to another (including segment history).Name and store comments with each generation (view).Unique Chronography feature tracks the history of any given DNA segment and maintains alternative views.Search, download, and import GenBank and EMBL files with all GenBank feature table items translated automatically into GCK features.Define thickness, pattern, shape, direction, and color for each DNA segment.Automatically monitor and adjust DNA fragment ends during cutting and pasting.View construct either as a text sequence or graphically, circular or linear.Manipulate actual DNA sequences through a unique and innovative graphical interface.Ideal for planning and tracking complex construction projects. A powerful productivity tool that no molecular biologist should be without.GCK was named the "Best Plasmid Mapping Software" by the Biotechnology Software and Internet Journal for four straight years. Gene Construction has an award winning interface design that allows graphic manipulation of DNA sequences in addition to providing sophisticated drawing capabilities for Mac and Windows users. Either method produces a panel showing matching entries, the features identified, and the source information contained within the field.The Gene Construction Kit allows graphic manipulation of DNA sequences and sophisticated plasmid drawing options. One can even generate a table output of 'No cutters' for a specific selection of DNA.įrom within Gene Construction Kit users can directly download a GenBank entry if an accession number is known - or one can search GenBank for keyword matches in all the relevant fields (Author, Gene Name, Journal, Organism, etc). Users can choose to mark enzyme sites by how many times they cut a sequence, by the kind of cut they create (5', 3', blunt), or even by whether or not they cut within a specified segment. Gene Construction Kit provides users with lists containing all known restriction enzymes with which to work, while also providing lists containing only commercial enzymes or enzymes sorted by recognition sequence lengths. Utilizing the special paste option allows a sequence to be inverted and/or to have the ends filled or trimmed before pasting, thus circumventing the ligation dialog if the manipulations warrant it. If such an attempt is made, GCK utilizes a unique 'ligation dialog' to assist users in adjusting the ends for compatibility. Incompatible ends cannot inadvertently be pasted together. Gene Construction Kit automatically monitors sticky ends that can be generated by restriction enzymes. When a selected segment is cut or copied, the ends are defined by the sites used to delimit the segment. Segments can be selected in either mode and cut, copied, or pasted. Users have complete control over the display in either mode. Gene Construction Kit is a biosoftware that graphically manipulates DNA.ĭNA can be represented and manipulated in either graphical or sequence/text modes - with any formatting or sequence manipulation changes made in one view, automatically being applied to the selection in the alternate view. (M/W) Updated drawing routines in Sequence and Construct windows.(M/W) Added extended support for Auto Annotation.(M/W) Resolved issue with AutoFeature sequence matches at 3' end of Construct.(M/W) Fixed junction marker Copy/Paste issue.(W) Addressed problems with printing Constructs under certain conditions.
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